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Mouse G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit

Mouse G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit

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Catalogue Number: ELK4544

Alternative Names: Glucose-6-phosphate 1-dehydrogenase

Species: Mouse

Assay Type: Sandwich

Standard: 20 U/L

Detection Range: 0.32-20 U/L

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research: Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase(G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase(G6PD) in the samples is then determined by comparing the OD of the samples to the standard curve.