Catalogue Number: ELK4544
Alternative Names: Glucose-6-phosphate 1-dehydrogenase
Species: Mouse
Assay Type: Sandwich
Standard: 20 U/L
Detection Range: 0.32-20 U/L
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase(G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase(G6PD) in the samples is then determined by comparing the OD of the samples to the standard curve.