Phospho-Histone H2AX-S139 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP0099-20, AP0099-100
Citations, Manuals and SDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Synonyms: H2A.X; H2A/X; H2AFX; Phospho-Histone H2AX-S139
GeneID: 3014
Swiss-Prot: P16104
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 15kDa
Observed Molecular Weight: 17kDa
Immunogen: A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P, IF/ICC
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:100 - 1:500 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:100 - 1:500
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Protein phosphorylation, ATM Signaling Pathway
Modifications: Phospho S139
Conjugate: Unconjugated
Positive Samples: NIH/3T3, C6
Cellular Location: Chromosome, Nucleus
Category: Phosphorylated Antibodies
Citations, Manuals and SDS Available upon request.