Phospho-Histone H3-S10 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP0002-20, AP0002-100
Citations, Manuals and SDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Phospho-Histone H3-S10
GeneID: 8290/8350
Swiss-Prot: Q16695/P68431
Reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Calculated Molecular Weight: 15kDa
Observed Molecular Weight: 15kDa
Immunogen: A synthetic phosphorylated peptide around S10 of human Histone H3 (P68431).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P, IF/ICC
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200
Research Areas: Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Cell Cycle, Immunology Inflammation, NF-kB Signaling Pathway
Modifications: Phospho S10
Conjugate: Unconjugated
Positive Samples: HeLa, NIH/3T3, C6
Cellular Location: Chromosome, Nucleus
Category: Phosphorylated Antibodies
Citations, Manuals and SDS Available upon request.