POLR2A (N-terminal) Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A21980-20, A21980-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Synonyms: RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; POLR2A (N-terminal)
GeneID: 5430
Swiss-Prot: P24928
Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB, IP, ChIP
Calculated Molecular Weight: 217kDa
Observed Molecular Weight: 250kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human POLR2A POLR2A (N-terminal) (NP_000928.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Protein phosphorylation, Neuroscience, Neurodegenerative Diseases
Recommended Dilutions: WB: 1:1000 - 1:5000 | IP: 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells | ChIP: 5μg antibody for 10μg-15μg of Chromatin
Modifications: Unmodified
Conjugate: Unconjugated
Cellular Location: Nucleus
Positive Samples: Mouse thymus
Keywords: 5430, sc-5943, sc-55492, sc-900, sc-899, sc-9001, POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, polymerase (RNA) II subunit A, P24928, RNA Polymerase II Subunit A, RNA Polymerase II Subunit B1, DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit, Polymerase (RNA) II (DNA Directed) Polypeptide A, DNA-Directed RNA Polymerase III Largest Subunit, RNA-Directed RNA Polymerase II Subunit RPB1, DNA-Directed RNA Polymerase II Subunit A, DNA-Directed RNA Polymerase II Subunit RPB1, Polymerase (RNA) II Subunit A, EC 2.7.7.48, EC 2.7.7.6, HRPB220, HsRPB1, Pol II, sc5943, sc 5943, sc55492, sc 55492, sc900, sc 900, sc899, sc 899, sc9001, sc 9001, POLR2A (N-terminal)
Symbol: POLR2A
Category: Monoclonal Antibodies
Research Use Only