Recombinant Human Wnt3a/Wnt-3a
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033972-10, PKSH033972-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Wnt3a; Wnt-3a
Target Synonym: MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family; member 3A; Wnt3a; Wnt-3a
UNIProt ID: P56704
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: None
Accession: P56704
Background: Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Sequence: Ser19-Lys352
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 10Mm PB .5% Sucrose, 0.01% Tween80 pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 105.9 kDa
Observed MW: 110-155 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only