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Recombinant Multidrug Efflux Pump Subunit ACRB - 10 µg

Recombinant Multidrug Efflux Pump Subunit ACRB - 10 µg

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CALIXAR’s AcrB transporter (Acriflavine resistance Protein B) is a bacterial multidrug exporter that aids in the identification, development, refinement, and validation of new molecules and therapeutic antibodies. Our AcrB transporters are exemplary for therapeutic indications such as bacterial resistance to antibiotics.

Acriflavine resistance Protein B (AcrB) is a bacterial multidrug exporter. AcrB is a good target for indications of bacterial resistance. Protein has a C-terminal 6X His tag.

Target name: Multidrug efflux pump subunit AcrB

Catalogue number: PP4

Class: Multidrug efflux pump

Sequence: Full-length, wildtype sequence, with a C-terminus, 6xHis-tag

Affinity Tag​: His-tag (C-terminal)

Origin: Escherichia coli (strain K12)

Theor. MW: 114.6 kDa

Accession #: P31224 (UniProt)​

Shipment temperature: Dry Ice

Storage conditions: Store at -80°C​

Expression system: Escherichia coli ( BL21C43)

Purity: >95%

Purification: Immobilized Metal Affinity Chromatography

Activity: Confirmed by ligand binding

Concentration​: Up to 5mg/ml

Sample buffer: 25mM Na2HPO4, 100mM NaCl, 0.01% DDM

Available quantity: From 10µg up to mg scale 

 

 

CALIXAR employs a novel & custom-built method 

CALIXAR’s AcrB transporters enable reliable fragment-based drug design (FBDD), structure-based drug discovery (SBDD) and antibody discovery against this specific target.

Unlike CALIXAR’s AcrB transporters, other alternative strategies result in an AcrB transporter that becomes mutated and truncated.

As with all transporters, AcrB transporters are delicate targets and are difficult to manufacture natively without particular know-how. Traditionally, AcrB transporters were frequently developed in a solution that inherently cannot be pure, neither native (truncated, mutated), and consequently very unstable.

With CALIXAR’s expertise, we are capable of producing AcrB transporters that preserve the native structure and function of the target. Our AcrB transporters are pure, native, unaltered and stable. This is unparalleled on the market and at the vanguard of Membrane Protein technology. 

 

CALIXAR’S AcrB transporters are unaltered, unmutated and untruncated targets that sustain their structural and functional integrity. Our AcrB transporters are purified and stabilized full length and wild-type (native) proteins and are produced in a prokaryotic arrangement (E.coli).

 

Calixar’s AcrB transporters are used for (bio)drug discovery and are adapted for application in Pharmaceutical, biotechnology companies, as well as for academic teams that are involved in the life science disciplines.

  • Antibodies (including nanobodies, scaffold proteins, aptamers)
  • Small molecules
  • 3D Structures (classical X-ray or XFEL, Cryo-EM, NMR)
  • Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
  • Antibody discovery (Immunization and display technologies)
  • Clinical stage (drug validation on reliable native AcrB)​

References


PROC NATL ACAD SCI USA

Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop.

Eicher T. et al. 2012 

 

ACTA CRYSTALLOGR BIOL CRYSTALLOGR

Purification, crystallization and preliminary diffraction studies of AcrB, an inner-membrane multi-drug efflux protein.

Pos KM. et al. 2002