Improving Low Expressed Antigen Detection in Immunofluorescence Applications

We are excited to introduce to you a silica nanoparticle technology from our partners AcZon. AcZon designed these silica nanoparticles with hopes of addressing the following challenges in Immunofluorescence:

 

  • Molecular fluorophores, such as FITC and RPE, show a rapid decay under stressing conditions
  • Often, the time needed by the operator to focus the samples and take pictures results in the bleaching of the fluorescent molecules and consequent signal intensity loss
  • For multiple tandem dyes, the drawback lies in the high instability of the acceptor molecules (usually cyanines)

 

AcZon’s silica nanoparticle provide a protective core/shell structure, protecting the sensitive fluorescent molecules increasing the fluorophores stability.

 

 

Key features:

  • The sensitive fluorescent molecules are covalently bound inside the silica matrix, providing shield effects
  • The external PEGylated shell allows water solubility and the anchoring of targeting biomolecules such as antibodies/peptides
  • The silica-nanoparticles entrap a higher number of fluorescent molecules, resulting in a higher signal

 

These features cause the fluorescence signal to be brighter and to last much longer than traditionally used molecular fluorochromes

 

Here we demonstrate the increased stability of nanoparticles-based fluorochromes (NTB575 and NTB530) with respect to traditionally used molecular fluorochromes (RPE and FITC):

 

 

 

Please reach out to us for a copy of the application note with full details.