Mouse Chia (Acidic mammalian chitinase) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9754-48, ELK9754-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Acidic mammalian chitinase [Precursor], Acidic mammalian chitinase, AMCase, Chia, CHIA_HUMAN, CHIT2, chitinase, acidic, DKFZp313J1722, ECF L, Lung specific protein TSA1902, Lung-specific protein TSA1902, RP5 1125M8.1, TSA1902
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Immunology, Microbiology, Signal Transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Chia. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Chia. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Chia, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Chia in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: Q91XA9
Research Use Only