Rabbit FASN (Fatty Acid Synthase) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9790-48, ELK9790-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase
Species: Rabbit
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Enzyme and Kinase,
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit FASN in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: P07855
Research Use Only