Chitozen - Functionalized Microscope Coverslips for Bacteria Live Imaging - TMI-CHI

Chitozen is a chitosan-coated coverslip immobilizing bacteria for microscopy without inducing bacteriostatic effects. It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to: 

• Image bacteria both still and alive under the microscope
• Maintain bacteria in a same focal plane for imaging while preserving their physiology 
• Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope

Sizes:

• 1 slide and 1 sticky slide per pack (TMI-CHI-1)
• 5 slides only (TMI-CHI-7525-min)
• 5 slides and 5 sticky slides per pack (TMI-CHI-7525)
• 1 slide, 1 sticky slide and 4 elements for centrifugation kit (TMI-CHI-1-CENTRI)
• 5 slides, 5 sticky slides and 4 elements for centrifugation kit (TMI-CHI-FULLPACK) Contact us for this product

Catalogue Numbers:

• TMI-CHI-1 (1 slide and 1 sticky slide per pack)
• TMI-CHI-7525-min (5 slides only)
• TMI-CHI-7525 (5 slides and 5 sticky slides per pack )
• TMI-CHI-1-CENTRI (1 slide, 1 sticky slide and 4 elements for centrifugation kit )
• TMI-CHI-FULLPACK (5 slides, 5 sticky slides and 4 elements for centrifugation kit) Contact us for this product

Applications

• Assay compatibility: 
  > Fluorescence
  > Co-cultures (bacterial predators, immune cells)
  > Addition of external factors (e.g. antibiotics, chemicals, inhibitors)
  > Static or dynamic conditions
• Imaging modes: 
  > phase-contrast, 
  > epifluorescence, 
  > confocal, 
  > super-resolution microscopy, 
  > atomic force microscopy (AFM) - documentation & example pictures available on request
• Experimental outputs:
  > Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, etc
  > Single molecule imaging 
• Chitozen is efficient with the following bacteria: 
  > E. coli
  > Bacillus subtilis
  > Caulobacter crescentus
  > Corynebacterium glutamicum
  > Helicobacter pylori 
  > Mycobacterium smegmatis
  > Myxococcus xanthus
  > Pseudomonas aeruginosa
  > Pseudomonas fluorescens
  > Salmonella
  > Staphylococcus aureus
  > Vibrio cholerae
  This list is updated regularly according to feedback provided by researchers who use Chitozen.

Kit contents

Chitozen coverslips are compatible with 6-channel sticky slides to work in a closed system in static or dynamic flow conditions. Order you coverslips alone if you need to work in an open system (i.e. for AFM imaging). 

• 5-coverslips alone*
  > 5x standard (25 x 75 mm) chitosan-coated coverslips
  
  
• 5-coverslips with microfluidic channels (30 experiments)
  > 5x standard (25 x 75 mm) chitosan-coated coverslips
  

> 5x bottomless 6-channel sticky slides

•  (Optional) centrifugation pack: the Chitozen coverslips can be centrifuged using a rack compatible with standard microscope slide (25mmx75mm) dimensions. If needed, add our centrifugation pack to your Chitozen kit. It includes: 

          > µ-Slide Microscopy Rack (ibidi
          > Magnetic Lid for Microscopy Rack
          > Clamp & adapter for sticky slides 

*If using the Chitozen coverslips alone, separate wells can be created manually by using a sealing glue or Stencell silicon chambers. We can provide these 2 products for you. Contact us for more information.

For custom orders (i.e. alternative coverslip amounts), please contact us. 

Lifetime: up to 12 months at room temperature, shielded from direct sunlight

Results: