ACAT1 Rabbit Monoclonal Antibody
Catalogue Numbers: MB66308-50, MB66308-100
Sizes: 50µl, 100µl
Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Swiss-Prot: P24752
Host: Rabbit
Reactivity: Human
Applications: WB, IP
All Applications: WB (1/500 - 1/1000), IP (1/10 - 1/50)
Background: Mitochondrial acetyl-coenzyme A (CoA) acetyltransferase 1 (ACAT1) plays a pivotal role in ketogenesis and branched chain amino acid metabolism. Research studies have demonstrated that ACAT1 also plays a key role in carbohydrate metabolism of tumor cells by directly acetylating and inhibiting the activity of the pyruvate dehydrogenase complex (PDH) and PDH phosphatase, which leads to decreased carbon flux through PDH and increased glycolysis. Mechanistically, it has been shown that numerous oncogenic tyrosine kinases directly phosphorylate ACAT1 at Y407, which promotes tetramerization and stabilization of the active enzyme in order to drive glycolysis and tumor growth.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of ACAT1 protein.
Extra Notes: Western blot analysis of ACAT1 expression in K562 (A), Hela (B) whole cell lysates.
Bioworld Molecular Weight: ~ 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: ACAT; MAT; Acetyl-CoA acetyltransferase mitochondrial; Acetoacetyl-CoA thiolase; T2
Immunogen: A synthetic peptide of human ACAT1
Conjugate: Unconjugated
Modification: Unmodified