Human IgG4 (Fc) Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A23201-20, A23201-100
Citations, Manuals and SDS Available upon request.
Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment).
Synonyms: IGHG4; Ig gamma-4 chain C region; Human IgG4 (Fc)
Swiss-Prot: P01861
Reactivity: Human
Calculated Molecular Weight: 36kDa
Observed Molecular Weight: 55kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 96-115 of Human IgG4 (Fc). (P01861).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IF/ICC
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:1000 - 1:5000 | IF/ICC: 1:100 - 1:500
Research Areas: Immunology & Inflammation
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: Human plasma, HeLa(Negative control)
Cellular Location: Secreted
Category: Monoclonal Antibodies
Citations, Manuals and SDS Available upon request.