Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP1283-20, AP1283-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Synonyms: AMPKa1/AMPKa2; Phospho-AMPKa1-T183/AMPKa2-T172
GeneID: 5562/5563
Swiss-Prot: Q13131/P54646
Reactivity: Mouse, Rat
Calculated Molecular Weight: 64kDa/65kDa/62kDa
Observed Molecular Weight: 62kDa
Immunogen: A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000
Research Areas: Protein phosphorylation
Modifications: Phospho T183/T172
Conjugate: Unconjugated
Positive Samples: C2C12+oligomycin, C6+AICAR
Cellular Location: apical Plasma Membrane, Cytoplasm, Cytosol, Nuclear Speck, Nucleoplasm, Nucleus
Category: Polyclonal Antibodies
Citations, Manuals and SDS Available upon request.