IL-2 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB67037-50, MB67037-100
Product: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.
Swiss-Prot: P60568
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation . IL-2 also promotes peripheral development of regulatory T cells (Tregs) . Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells . The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc . IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ . γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 . Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways .
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of IL-2 protein.
Bioworld Molecular Weight: ~ 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of IL-2 expression in K562 (A) whole cell lysates., ???, ???, ???
Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-2. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified