Recombinant Full Length Human TRIM25 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
Size: 20ug
Catalogue Number: TRIM25-483HFL
Description: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Source: Mammalian cells
Species: Human
Tag: Flag
Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Mass: 70.8 kDa
AA Sequence: MAELCPLAEELSCSICLEPFKEPVT TPCGHNFCGSCLNETWAVQGSPYLC PQCRAVYQARPQLHKNTVLC NVVEQFLQADLAREPPADVWTPPAR ASAPSPNAQVACDHCLKEAAVKTCL VCMASFCQEHLQPHFDSPAF QDHPLQPPVRDLLRRKCSQHNRLRE FFCPEHSECICHICLVEHKTCSPAS LSQASADLEATLRHKLTVMY SQINGASRALDDVRNRQQDVRMTAN RKVEQLQQEYTEMKALLDASETTST RKIKEEEKRVNSKFDTIYQI LLKKKSEIQTLKEEIEQSLTKRDEF EFLEKASKLRGISTKPVYIPEVELN HKLIKGIHQSTIDLKNELKQ CIGRLQELTPSSGDPGEHDPASTHK STRPVKKVSKEEKKSKKPPPVPALP SKLPTFGAPEQLVDLKQAGL EAAAKATSSHPNSTSLKAKVLETFL AKSRPELLEYYIKVILDYNTAHNKV ALSECYTVASVAEMPQNYRP HPQRFTYCSQVLGLHCYKKGIHYWE VELQKNNFCGVGICYGSMNRQGPES RLGRNSASWCVEWFNTKISA WHNNVEKTLPSTKATRVGVLLNCDH GFVIFFAVADKVHLMYKFRVDFTEA LYPAFWVFSAGATLSICSPKTRTRP LEQKLISEEDLAANDILDYKDDDDK V
Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage: Store at -80 centigrade.
Concentration: >50 ug/mL as determined by microplate BCA method.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families: Druggable Genome, Transcription Factors
Protein Pathways: RIG-I-like receptor signaling pathway