High-efficiency proteofection reagent for mammalian cells
Catalogue Numbers:
- E010-0.1 (1 x 100 µl)
- E010-0.25 (1 x 250 µl)
Sizes:
- 1 x 100 µl (E010-0.1)
- 1 x 250 µl (E010-0.25)
HIGHLIGHTS:
- High efficiency
- Broad range of application with both proteins and cells
- Ultra-low toxicity – cell activity is maintained
- Full serum compatibility
- Efficient and rapid protocol: proteofection within 6 hours
Product Specification
| Application | Proteofection of mammalian cells with proteins |
|---|---|
| Content | PROTEOfectene®, 100 µl R-Phycoerythrin (100 µg/ml) as positive control |
| Assays | Approx. 100 (24-well) or approx. 20 (6-well) per 100 µl reagent |
| Shipping | At room temperature |
| Storage | 4°C |
| Shelf life | 1/2 year (from date of delivery) |
| Product Group | Proteofection |
PROTEOfectene® enables proteins to be introduced directly into mammalian cells without the need for prior gene expression.
Proteins introduced with PROTEOfectene® retain their structure and function completely unchanged. This opens new possibilities, from localization studies using fluorescence-marked proteins to direct manipulation of cell metabolism by means of functionally capable enzymes.
Some major features of PROTEOfectene® are its high efficiency, low toxicity and serum compatibility, making it a unique addition to the range of protein transport reagents. R-Phycoerythrin is a very suitable red fluorescent protein for application as positive control in proteofection experiments.
Proteofection of CHO-K1 cells with β-galactosidase and PROTEOfectene®. The subsequently fixed and dyed slide shows the functional capability of the enzyme introduced.
Proteofection of BEAS-2B cells with R-phycoerythrin and PROTEOfectene®.
Proteofection of Hep G2 cells with R-Phycoerythrin
Proteofection of Hep G2 cells by using the µ-Proteofection Kit VI. Pictures were recorded 24h after addition of the PROTEOfectene/R-Phycoerythrin complex:
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Picture of cells |
Fluorescence picture: Most bright spots are cell fragments loaded with R-Phycoerythrin. Note the fluorescent cytoplasmas and nonluminous cell nuclei. |
Proteofection of HeLa cells with GST-NLS-GFP protein:
Proteofection of HeLa cells by using µ-Proteofection Kit VI. Pictures were recorded 24h after addition of the PROTEOfectene®/GST-NLS-GFP complex:
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Picture of cells |
Fluorescence picture. Move cursor over the picture for viewing the overlay picture: Cell nuclei have quantitatively imported the NLS-GFP protein. |