Pro-DeliverIN™ Transfection Reagent is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid-based formulation is the first serum compatible reagent to deliver functional proteins into living cells. The proteins delivered inside cells retain their biological functions.
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Intracellular delivery of functionally active proteins
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Highly efficient in many primary cells and cell lines
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Serum compatible
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Biodegradable and non-toxic
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Easy: straightforward protocol and ready-to-use (no chemical conjugation required).
Pro-DeliverIN™ is provided with 100µL of R-Phycoerythrin Positive Control.
Sizes:
- 100µL (PI10100): 50-100 assays
- 250µL (PI10250): 125-250 assays
- 500µL (PI10500): 250-500 assays
- 1 mL (PI11000): 500-1000 assays
Shipping conditions: Room temperature
Application
- Suitable for primary cells and cell lines
- Ideal for your protein delivery applications:
- Intracellular localization studies in living cells
- Protein-protein interactions
- FRET studies
RECOMMENDED FOR: Intracellular delivery of proteins.
Results
Figure 1 : Intracellular delivery of R-phycoerythrin into various cell lines. 1 µg of R-Phycoerythrin was mixed with 2 µL of Pro-DeliverIN™ reagent. Complexes were then incubated with different cell lines in 24-well plates. Living cells were observed 24h later by fluorescence microscopy.
Figure 2: Pro-DeliverIN delivers fully functional proteins. HeLa cells were treated with 15 ng of active human caspase-3 and 5 µL of the Pro-DeliverIN reagent in 24-well plates. As controls, cells were treated either with 15ng of caspase 3 alone, 5 μL of Pro-DeliverIN alone or 100 nM staurosporine (positive control). After 7h of incubation, cells were stained with Annexin-FITC and propidium iodide. Apoptotic and dead cells were monitored by cytofluorimetry.
GENOME EDITING
Pro-DeliverIN succeeded in transporting active CRE recombinase that induced homologous recombination consequently.
Homologous Recombination: HEK-293 cells were stably transfected with GFP plasmid containing LOX sequences. Active CRE recombinase Enzyme was delivered using Pro-DeliverIN reagent and GFP expression was monitored 96 H after delivery.