Human COL2 (Collagen Type II) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK2618-48, ELK2618-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Collagen Type 2; Col 2; Collagen Type II; Col II
Species: Human
Assay Type: Sandwich
Sensitivity: 37.24 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, Plasma, Tissue Homogenates and other Biological Fluids
Assay length: 3.5h
Research Areas: Metabolic pathway; Developmental science; Hepatology; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.