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Magnetofectamine O2 Transfection Kit
Magnetofectamine O2 Transfection Kit
Magnetofectamine O2 Transfection Kit

Magnetofectamine O2 Transfection Kit

Regular price
$611.16 CAD
Regular price
Sale price
$611.16 CAD
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Magnetofectamine™ O2 is the new powerful and ideal transfection kit designed for primary and hard-to-transfect cells. The alliance of MTX transfection reagent and CombiMag leads to increased transfection efficiency, minimized toxicity and enhanced gene expression.

This kit replaces and outperforms the original Magnetofectamine which was the combination of Lipofectamine™* 2000 from Life Technologies Corporation and CombiMag Transfection Reagent.

CombiMag, a magnetic formulation based on the Magnetofection™ technology, binds to MTX transfection reagent/DNA complexes and under the application of a magnetic field concentrates the genetic material onto cells and promotes cellular uptake. In this way, transfection efficiency is enhanced.

  • Less nucleic acids used - minimized toxicity
  • No need to change your standard protocol
  • Ideal for hard-to-transfect and primary cells
  • Simple, ready-to use and rapid
  • Serum compatible and applicable to all nucleic acids

Magnetofectamine O2 Transfection Kit for Primary Cells

Sizes 
  • MTX2-1000 Starting Kit: 1 super Magnetic Plate (MF1000) + 250 µL CombiMag + 750 µL MTX reagent + 3 ml MTX Boost 100X
  • MTX2-0750: 250 µL CombiMag + 750 µL MTX reagent + 3 ml MTX Boost 100X
 
Storage : Upon reception and for long-term use, store the MTX REAGENT and MTXBoost at -20°C and CombiMag at +4°C.
Shipping Conditions : Room temperature
 
*Lipofectamine™ and Invitrogen™ are Trademarks owned by Life Technologies Corporation.
  

 

Applications

Magnetofectamine O2 Transfection Kit for Primary Cells

  • Boost transfection efficiency with reduced cell toxicity
  • Ideal for mammalian cells: Cell lines, primary & hard to transfect cells.
  • Perfect for all transfections: Transient, stable, gene silencing, with or without serum
  • Suitable for all nucleic acids: DNA, oligonucleotides, mRNA, siRNA, shRNA

 

RECOMMENDED FOR: Transfection of primary and hard-to-transfect adherent cells 

 

Results

Figure 1: Magnetofectamine O2 transfection efficiency.

 

Magnetofectamine Transfection efficiency

Figure 2:  As the magnetic force drives the gene vector towards the target cells, Magnetofectamine™ O2 allows the vector dose to concentrate onto the cell very rapidly and triggers delivery via endocytosis. Consequently, high transfection efficiencies can be achieved with less nucleic acid amount.