Pro-DeliverIN™ Transfection Reagent is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid-based formulation is the first serum compatible reagent to deliver functional proteins into living cells. The proteins delivered inside cells retain their biological functions.
Intracellular delivery of functionally active proteins
Highly efficient in many primary cells and cell lines
Biodegradable and non-toxic
Easy: straightforward protocol and ready-to-use (no chemical conjugation required).
Pro-DeliverIN™ is provided with 100µL of R-Phycoerythrin Positive Control.
- 100µL (PI10100): 50-100 assays
- 250µL (PI10250): 125-250 assays
- 500µL (PI10500): 250-500 assays
- 1 mL (PI11000): 500-1000 assays
Shipping conditions: Room temperature
- Suitable for primary cells and cell lines
- Ideal for your protein delivery applications:
- Intracellular localization studies in living cells
- Protein-protein interactions
- FRET studies
RECOMMENDED FOR: Intracellular delivery of proteins.
Figure 1 : Intracellular delivery of R-phycoerythrin into various cell lines. 1 µg of R-Phycoerythrin was mixed with 2 µL of Pro-DeliverIN™ reagent. Complexes were then incubated with different cell lines in 24-well plates. Living cells were observed 24h later by fluorescence microscopy.
Figure 2: Pro-DeliverIN delivers fully functional proteins. HeLa cells were treated with 15 ng of active human caspase-3 and 5 µL of the Pro-DeliverIN reagent in 24-well plates. As controls, cells were treated either with 15ng of caspase 3 alone, 5 μL of Pro-DeliverIN alone or 100 nM staurosporine (positive control). After 7h of incubation, cells were stained with Annexin-FITC and propidium iodide. Apoptotic and dead cells were monitored by cytofluorimetry.
Pro-DeliverIN succeeded in transporting active CRE recombinase that induced homologous recombination consequently.
Homologous Recombination: HEK-293 cells were stably transfected with GFP plasmid containing LOX sequences. Active CRE recombinase Enzyme was delivered using Pro-DeliverIN reagent and GFP expression was monitored 96 H after delivery.